Crude enzyme extract was subjected to heat treatment at 40-80°C for 0-60 min. The optimum pH of the enzyme is 7.1, the most stable pH range is 3.9-4.2, and the optimum reaction temperature is 55°C. (Ind. Fruit bromelain (FBM) appears to have higher activity at optimum temperature of 45ºC than SBM (optimum temperature of 35 ºC). bromelain with anhydride was obtained at 60oC and high activity was observed at incubation temperature between 50oC to 70oC. Independent variable: Temperature Dependent variable: Bromelain activity. Although it is present in all parts of the pineapple plant, the stem is the most bromelain rich part that also makes it the most common commercial source of bromelain (Rowan, Buttle & Barrett, 1990, p.869-875) 1. 2.7.2. Optimum activities with casein as substract were observed corresponding to temperatures ranging from 10 to 20°C at acidic pH values, from 30 to 40°C at basic pH values, and from 40 to 60°C at neutral pH values (pH 6.8 ÷ 7.1). Bromelain: Enzyme that breaks down protein into Amino Acids. Mg 2+ and Ca 2+ have an inhibitory effect on bromelain activity at high concentrations, but have a promoting effect at low concentrations. Fresh pineapple juice: Source of bromelain. 2012;Silvestre et … Independent variable: pH Dependent variable: Bromelain activity. The temperature stability profiles as a function of different time intervals showed higher retention of enzyme activity at low temperature. & Dep.) Both pH and temperature significantly had effects on fruit bromelain activities. Like temperature, pH, or the relative measure of acicity (pH = 0 to 6.99) or alkalinity (pH = 7.1 to 14) of a solution, will greatly influence the activity of most enzymes. Effects of Temperature, pH, and Gelatin on BromelainIntroduction Bromelain is an organic catalyst that speeds up the rate of chemical reactions. The crude aqueous extract from the pineapple fruit is known as bromelain. Bromelain is used for … Enzymes enable … It has a centuries-long history of being used to … The optimum temperature of recBM and cBM was determined by using ZPVANA as the substrate under its determined optimum pH. Bromelain is a protein-digesting enzyme mixture derived from the stem, fruit, and juice of the pineapple plant. Incubation at 40 °C showed People sometimes use it as a medicine. Bromelain is used in this experiment to catalyze the break down of proteins and is called a proteolytic enzyme. Effect of pH on bromelain activity. Bromelain is a group of enzymes found in pineapple juice and in the pineapple stem. Determination of Bromelain Activity at Different Temperature . Fruit bromelain was extracted from the fruit of pineapples. Early reports indicated that the optimal pH for bromelain proteolytic activity was close to 7, or neutral pH. Bromelain is a protein extract present in the fruit, leaves and stems of pineapple plants (Ananas comosus). Although the optimum temperature for the highest bromelain activity appeared to be in the range between 50 and 70°C (Ketnawa et al.